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A Single Chamber Microbial Fuel Cell (MFC) has been fabricated to generate electricity from the sludge of the sewage treatment plant at Anand International College of Engineering, Jaipur, at two different ambient temperature range of 25 ± 4°C and 32 ± 4°C under aerobic condition. The maximum voltage obtained was about 2890 mV after 80 (hrs.) at temperature range of25 ± 4°C, with the surface power density of 1108.29 mW/m2. When the ambient temperature was 32 ± 4°C, the maximum voltage obtained was 1652 mV after 40 (hrs.) surface power density reduced to 865.57 mW/m2.While changing the amount of substrate for certain area of the electrode at 25 ± 4°C range it showed that the electricity generation decreased with the available substrate and it also shortens the time to peak voltage. On the other way when the ambient temperature increased to 32 ± 4°C, the maximum potential energy generated is less than the previous temperature for the same substrate per unit area of the electrode and also the time to peak voltage decreases to 40 hrs. At the end of the 152 (hrs.), the maximum COD reduction for the sewage sludge was 30% for 32 ± 4°C. When comparing with other single chambers MFC, the present model is generating more electricity that any MFC using sewage sludge as substrate except platinum electrode, which is much costlier that electrode used in the present study.