Biography

Gaoyun Chen published latest article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences entitled Quantification of amlodipine in dried blood spot samples by high performance liquid chromatography tandem mass spectrometry. This article is available in PubMed with an unique identification number PMID: 29195144 and it is published in 2018. The coauthors of this article are Chen G; Jirjees F; Al Bawab A; McElnay JC.


Research Interest

Pharmacy


Latest Publication Details

Article Title: Quantification of amlodipine in dried blood spot samples by high performance liquid chromatography tandem mass spectrometry.

Co-Author(s): Chen G; Jirjees F; Al Bawab A; McElnay JC

Affiliation(s): Clinical and Practice Research Group, School of Pharmacy, Queens University Belfast, 97 Lisburn Road, Belfast BT9 7BL, UK.

PMID 29195144, Year 2018

Abstract: A sensitive and specific method, utilising high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) was developed for the quantitative determination of amlodipine in dried blood spot (DBS) samples. Chromatographic separation was achieved using a Waters XBridge C18 column with gradient elution of a mixture of water and acetonitrile containing 0.1% formic acid (v/v). Amlodipine was quantified using a Waters Quattro Premier mass spectrometer coupled with an electro-spray ionization (ESI) source in positive ion mode. The MRM transitions of 408.9?m/z?238.1m/z and 408.9?294.0?m/z were used to quantify and qualify amlodipine, respectively. The method was validated across the concentration range of 0.5-30ng/mL by assessing specificity, sensitivity, linearity, precision, accuracy, recovery and matrix effect according to the Food and Drug Administration (FDA) guidelines. This method was also validated clinically within a large pharmacoepidemiological study in which amlodipine blood concentration was determined in patients who had been prescribed this medication.

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

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