Biography

Catriona P Harkins published latest article in The Journal of investigative dermatology entitled The Microevolution and Epidemiology of Staphylococcus aureus Colonization during Atopic Eczema Disease Flare. This article is available in PubMed with an unique identification number PMID: 28951239 and it is published in 2018. The coauthors of this article are Harkins, CP; Pettigrew, KA; Oravcov?, K; Gardner, J; Hearn, RMR; Rice, D; Mather, AE; Parkhill, J; Brown, SJ; Proby, CM; Holden, MTG.


Research Interest

Veterinary Science


Latest Publication Details

Article Title: The Microevolution and Epidemiology of Staphylococcus aureus Colonization during Atopic Eczema Disease Flare.

Co-Author(s): Harkins, CP; Pettigrew, KA; Oravcov?, K; Gardner, J; Hearn, RMR; Rice, D; Mather, AE; Parkhill, J; Brown, SJ; Proby, CM; Holden, MTG

Affiliation(s): School of Medicine, University of St Andrews, St Andrews, UK; Department of Dermatology, Ninewells Hospital, Dundee, UK; School of Medicine, University of Dundee, Dundee, UK. Electronic address: ch246@st-andrews.ac.uk.

PMID 28951239, Year 2018

Abstract: Staphylococcus aureus is an opportunistic pathogen and variable component of the human microbiota. A?characteristic of atopic eczema (AE) is colonization by S.?aureus, with exacerbations associated with an increased bacterial burden of the organism. Despite this, the origins and genetic diversity of S.?aureus colonizing individual patients during AE disease flares is poorly understood. To examine the microevolution of S.?aureus colonization, we deep sequenced S.?aureus populations from nine children with moderate to severe AE and 18 non-atopic children asymptomatically carrying S.?aureus nasally. Colonization by clonal S.?aureus populations was observed in both AE patients and control participants, with all but one of the individuals carrying colonies belonging to a single sequence type. Phylogenetic analysis showed that disease flares were associated with the clonal expansion of the S.?aureus population, occurring over a period of weeks to months. There was a significant difference in the genetic backgrounds of S.?aureus colonizing AE cases versus controls (Fisher exact test, P?= 0.03). Examination of intra-host genetic heterogeneity of the colonizing S.?aureus populations identified evidence of within-host selection in the AE patients, with AE variants being potentially selectively advantageous for intracellular persistence and treatment resistance.

Journal: The Journal of investigative dermatology

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