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The Detection of Bornavirus in Birds, Mammals and Snakes by Sandwich ELISA of Bornaviral Matrix Protein

Author(s): Josephine Marie McHugh, Siwo Rel de Kloet

This study describes a further analysis of the detection of bornaviral antigens by sandwich ELISA. The results show that using chicken anti-psittacine bornaviral P16 matrix protein antibody for capture and rabbit antibody to this protein for detection, recombinant matrix protein of mammalian as well as of distantly related snake bornavirus can be detected, suggesting that a single procedure can be used for the diagnosis of highly divergent bornavirus infections.

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