Anticholinesterase Properties of Mareya Micrantha (Benth) Mull. Arg. (Euphorbiaceae) and its Fractions on the Cholinesterase Model of Isolated Rabbit Duodenum

Author(s): Mamadou Dosso, Tianga Yaya Soro, Michel Archange Libra, Flavien Traore, Jean David N’guessan

Abstract

The study of the anticholinesterase properties of Mareya micrantha, a plant used in African pharmacopoeia as a laxative, was carried out on cholinesterases of the rabbit duodenum. At 0.15 mg / ml, the aqueous extract (MAR), its fraction (F5) and its sub-fraction (F52), depending on their state of purity, decrease the catalytic speed of cholinesterases during the hydrolysis of the acetylthiocholine (ASCh), without significantly influencing the Michaelis constant. The sub-fraction (F52) with the best anti-cholinesterase and myostimulant effects revealed by the bio-guided study is 25% more effective than MAR. A study of the High performance liquid chromatography (HPLC) chromatogram of this sub-fraction F52 shows that this plant is rich in phenolic molecules.