Dose and Duration Dependent 2,3,7,8 TCDD (Dioxin) induced Enzymatic and Histopathological changes in the Liver Cells of Mice

Author(s): Jyoti Jigyasi, Aarti Chotaliya, Rahul Kundu

Abstract

2,3,7,8 TCDD, a Dioxin, is one of the most potent carcinogenic environmental persistent organic pollutants. The present investigation was aimed to assess the histopathological and glutathione enzymes alterations caused by TCDD in the liver cells of white albino mice. The hypotheses set to assess the histopathological alterations are, (a) whether sublethal doses of TCDD exposed to mice for sub-acute duration can alter the normal activity of the antioxidative enzymes in liver cells, and (b) whether dose and duration dependenrt exposure to TCDD can induce the histopathological lesions in the mice hepatocytes. Separate groups of mice were subjected to the oral doses of TCDD (4 μg/kg bw and 40 μg/kgbw/d) for 7, 14 and 21 days of exposure durations. Control group were administered corn oil (vehicle) separately. The obtained results suggested a predominant exposure duration dependent effects in the activities of the antioxidative enzymes. The results suggested that the observed alteration in activity of the antioxidative enzymes could be due to oxidative stress which might have been an indirect effect following TCDD exposure. The results also indicated that the TCDD also caused histopathological lesions in the hepatic cells. Few of the observed lesions were severe and occurred in the high doses exposed for maximum durations.