Genetic Diversity of Eristalis Tenax (Linnaeus, 1758) as Insect Pollinator of Prunus Persica (L.) Stokes Flowers Based on MtcoⅠ Gene

Author(s): Poonam Dhiman and Mahender Singh Thakur

Eristalis tenax is an important insect pollinator of Prunus persica plant and samples were collected from seven localities of Himachal Pradesh i.e. Bathra (503m), Jaladi (508m), Hamirpur (786m), Rajgarh (1555m), Naldera (1887m), Summer Hill (2100m) and Mashobra (2146m). Phylogenetic relationship and multiple sequence alignment of all the sampled sequences of Eristalis tenax were analyzed by using mtCOI gene. Nucleotide composition analysis showed that percentage of A+T (69.03%) was higher than the percentage of C+G (30.97%) which showed that all the sequences were AT biased. Multiple sequence alignment showed three variable sites in the sequences of Eristalis tenax and the comparable values of transitions and transversions in the current study suggest the possible occurrence of genetic divergence over evolutionary time scales in Eristalis tenax of Himachal Pradesh.