RAPD and ISSR Banding Pattern of Alpinia Galanga Populations from Eastern India

Author(s): Reena Parida and Sanghamitra Nayak

India is very well known for presence of medicinal aromatic plants for treatment of various diseases which are day by day diminishing from the nature. This requires an urgent need for conservation of germplasm of those high yielding varieties which arises to help in various ways to the pharmaceutical, cosmetic as well as other industries. So also their genetic study is very much essential in order to explore their molecular structure. The present study was undertaken to characterize Alpinia galanga collected from four different populations of Odisha using two molecular markers as Random Amplified Polymorphic DNA and Inter Simple Sequence Repeats. Further a dendrogram was constructed through sequential agglomerative hierarchical and nested clustering, unweighted pair group method with arithmetic mean analysis and Jaccard’s similarity coefficient of combined markers which segregated these genotypes into two main clusters moreover showing the genetic similarity between the germplasm. Hence, the molecular analysis could be further used for identification of important novel gene present in Alpinia galanga which can be utilized for future crop improvement.