Evaluation of the Effect of Pouteria Campechiana’s Fruit Powder and Ethanolic Extract on Aluminum-Chloride Induced Alzheimer’s Disease

Author(s): Joel Geradin Tueguem Tagne, Anne Pascale Nouemsi Kengne, Hermine Tsafack Doungue, Michel Pegui Kemtsop, Julius Oben Enyong

Objective: The aimed of this work was to assess the protective effect of Pouteria campechiana’s (PC) fruit on neurological disorder and oxidative stress induced by aluminium chloride.

Methods: Alzheimer disease were induced using 100 mg/kg of AlCl3 orally twice/week during the 42 days. The behavioural assessment was performed using Water maze and eight arms maze tests. The activity of pouteria campechiana fruit were evaluated by administration of 200 mg/kg, 400 mg/kg fruit ethanolic extracts and 5% and 10% of PC’s fruit powders respectively. Wistar rats were treated for 42 days along with aluminium chloride. After treatment, Biochemical estimation of oxidative stress parameter and acetylcholine activity was done in brain also, the Brain histopathological examination was done by microscopic observations.

Results: Aluminium chloride treatment results in memory impairment and oxidative stress while treatment with powders and ethanolic extracts of PC showed an improvement on reduction of this oxidative stress by lower behavioural impairment, MDA content and the increased of total proteins, reductive glutathione, acetylcholinesterase and catalase, Histopathological examination revealed the presence of amyloid plaques and neurofibrillary tangles in the brain of negative control rats. For those treated with PC’s powders and extracts, few or no hallmarks were present.

Conclusions: PC’s fruit ethanolic extracts and powders revealed a protective effect against neurodegenerative effects of aluminum-induced Alzheimer’s disease (AD). PC fruit may treat AD.