Revivify Modulates Healthy Gut Microbiomes and Short Chain Fatty Acids Evaluated by an in vitro model of Gut Microbiome study

Author(s): Ashraf AHMZ, Ahmed F Pantho, Samee Kamal, Alyssa Caba, Syeda H Afroze, Thomas J Kuehl, Liaquat Hossain, M. Nasir Uddin

Background: The diverse microbial community in our gastrointestinal (GI) helps in the fermentation of metabolites of Short Chain Fatty Acids (SCFAs), mainly acetate, propionate, butyrate, and small number of lactates. Acetate, propionate, and butyrate maintain colonic lining integrity and ideal colon activity. Butyrate is also known to be an energy source and assist in anti-inflammatory response. Maintaining an optimal ratio of these SCFAs reduce the risk of inflammation and promote a healthy colon. We evaluated the effect of Revivify Oral Liquid Gel (composition: US Patent 11 224 636) on gut microbiomes and SCFAs by an in vitro model of gut microbiome study. Gut microbes were cultured in 2 ml 96-well plates and treated with control, SOD, Prefibrotic fiber, Fruit juice, and Revivify gel for 48 hours followed by metaproteomic, chemical analysis (SCFA content), and microbiome profiling.

Results: We found that the amount of SCFAs increased by 2.5 folds, when treated with Revivify gel but the ratio of the SCFAs remain the same across all the treatment groups. Revivify gel promoted the growth of the Firmicutes spp., especially Lactobacillus, a probiotic organism.

Conclusions: Our study demonstrates that Revivify gel promotes a balanced increase of the SCFAs in a consistent manner, supporting a beneficial population of gut microbes for optimal gastrointestinal functionality.