Pharmacological Reinvention: Drug Repurposing Strategies to Tackle Aggressive High Grade Serous Ovarian Carcinoma (HGSOC)

Author(s): Faisal Iqbal

The poor prognosis and limited treatment option make high grade serous ovarian carcinoma (HGSOC) is an aggressive malignancy. Drug repurposing provides a cost effective and fast strategy to recognize novel treatment possibilities. In this study, investigated the individual and joint therapeutic potential of three repurposed drugs like auranofin, metformin and chlorpromazine (CPZ) in HGSOC. A potent effect for all three drugs was revealed, while triple combination of drugs screening is strongest reduction in cell proliferation. The triple combination of drug synergistic effect was more noticeable than single or double drug treatment. The findings recommend that the combination of auranofin, CPZ and metformin are helpful against HGSOC of OVCAR3 cell line. The hemocytometer and clonogenic assay were performed to evaluate the counting and survival cells in colonies form, further ANOVA and Tukey’s post-hoc test was run for statistical analysis and validated the significance of drug repurposing effects while after polymerase chain reaction (PCR) the gel electrophoresis was performed for molecular analysis. The study defines a novel pharmacological approach that repurposes existing drugs to achieve a better anti-cancer effectiveness in HGSOC.